Alanyl - tRNA synthetase gene of the extreme acidophi I ic chemol ithoautotrophic Thiobacillus ferrooxidans is highly homologous to alas
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چکیده
Laboratoire de Chirnie Bact&ienne, lnstitut de Biologie Structurale et de Microbiologie, 31 chemin Joseph Aiguier, 13402 Marseille C dex 2of France The alas gene of Thiobacillus ferrooxidans has been cloned and sequenced and its expression in Escherichia coli and T. fermoxidans analysed. The same genOmiC Organization to that in Em COli (I'ed-recX-ahS) has been found in T. ferrooxidans. The r e d and alas genes cannot be transcribed from their own promoters in E. coli. In addition to the well-known homology at the protein level between Alas proteins from various organisms, a strong homology was found between all the known alas genes from bacteria, archaea and eucarya. Two regions, one of which corresponds to the catalytic core, are particularly well-conserved at the nucleotide sequence level, a possible indication of strong constraints during evolution on these parts of the genes.
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